Characterization of two conformational epitopes of the Chlamydia trachomatis serovar L2 DnaK immunogen
نویسندگان
چکیده
منابع مشابه
Characterization of outer membrane proteins in Chlamydia trachomatis LGV serovar L2.
We used a photoactivatable, lipophilic reagent, 3'-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, to label proteins in the outer membrane of elementary bodies of Chlamydia trachomatis LGV serovar L2 and mass spectrometry to identify the labeled proteins. The identified proteins were polymorphic outer membrane proteins E, G, and H, which were made late in the developmental cycle, the major o...
متن کاملExpression, Processing, and Localization of PmpD of Chlamydia trachomatis Serovar L2 during the Chlamydial Developmental Cycle
BACKGROUND While families of polymorphic membrane protein (pmp) genes have been identified in several Chlamydia species, their function remains mostly unknown. These proteins are of great interest, however, because of their location in the outer membrane and possible role in chlamydial virulence. METHODOLOGY/PRINCIPAL FINDING We analyzed the relative transcription of the pmpD gene, a member o...
متن کاملInteraction of Chlamydia trachomatis serovar L2 with the host autophagic pathway.
Chlamydiae are obligate intracellular pathogens that replicate within a membrane-bound compartment (the inclusion) and are associated with important human diseases, such as trachoma, pneumonia, and atherosclerosis. We have examined the interaction of the host autophagic pathway with Chlamydia trachomatis serovar L2 by using the specific autophagosomal stain monodansylcadaverine, antibodies to a...
متن کاملSequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2.
The structural gene for the major outer membrane protein (MOMP) from Chlamydia trachomatis was cloned and sequenced. A lambda gt11 recombinant (lambda gt11/L2/33) that contains a portion of the MOMP coding sequence was used to probe a lambda 1059 library constructed from DNA obtained from C. trachomatis serovar L2. Selected lambda 1059 recombinants were mapped with endonuclease restriction enzy...
متن کاملSerovar distribution of urogenital Chlamydia trachomatis strains
The distribution of serovars in 208 Chlamydia trachomatis strains of urogenital origin isolated from 185 patients attending a sexually transmitted disease clinic in Rotterdam, The Netherlands, was assessed. Typing by monoclonal antisera using a dot enzyme linked immunosorbent assay (ELISA) showed that the most common serovars were E (found in 45 strains), F (39), D (34), and K (28). Other serov...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1996
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.64.3.810-817.1996